Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis
نویسندگان
چکیده
منابع مشابه
Oligonucleotide synthesis using the manual phosphotriester method.
During the past few years, synthetic DNA, used as a primer in DNA polymerization, in site-directed mutagenesis or as a probe in gene selection, has assumed a central role in recombinant DNA technology (1). This was made possible by the development of methods for efficient solid phase chemical synthesis. Deoxyribonucleotides are ideally suited to solid-phase synthesis since their relative chemic...
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چکیده ندارد.
comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.
heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولAn Efficient and One-pot Procedure for the Synthesis of Chiral Isoxazolidine via Catalytic Highly Enantioselective 1,3-dipolar cycloaddition
Synthesis of enantiomerically pure isoxazolidine via an asymmetric 1,3- dipolar cycloaddition reaction of nitrone with electron-deficient dipolarophile was described. The process occurs at room temperature in aqueous ethanol as a green solvent and in the presence of a bidendate bis(imine)–Cu(II)triflate complex as catalyst. The reaction mechanism is discussed on the basis of the assignment of t...
متن کاملHighly efficient photolabile protecting groups with intramolecular energy transfer.
DNA chips are widely used for genomic analysis. Currently, high-density DNA chips can be synthesized with up to several hundred thousand different spots on an area of 1 cm. In principle, the complete human genome can be probed with such a chip. The required high spot densities can be achieved by photolithographic in situ DNA-chip synthesis. In this method, the oligonucleotides are synthesized s...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.16.6525